Ponentes
Descripción
Abstract: Peptide-based cancer therapy has unique advantages. CIGB-552 is a synthetic peptide that has been shown to be effective in reducing tumor size in tumor-bearing mice. Its anticancer effects are due to its ability to penetrate cells and induce a pro-apoptotic effect and modulate the NF-kB signaling pathway and the angiogenesis process through its main mediator, the COMMD1 protein. Previous experiments showed that there was a relationship between the sensitivity of the cell line and the ability of cell penetration, but the penetration kinetics and intracellular permanence in tumor cells were not described. Our objective was the detection of the intracellular penetration capacity of the CIGB-552 peptide in the H460, HCT-116 and HT-29 tumor cell lines. Fluorescence microscopy and flow cytometry techniques were used. The CIGB-552 peptide is capable of penetrating the 3 cell lines studied: H460, HCT-116 and HT-29, with maximum intracellular accumulation at 2 hours in the first, and 1 hour in the last two. The higher cytotoxic activity in the H460 cell line could be related to its higher capacity to capture the CIGB-552 peptide. The maximal accumulation of CIGB-552 in the H460 cell line is in accordance with the kinetics of the main molecular events described above.
Keywords: cell penetration, stability, kinetics, cell lines, CIGB-552
Bibliography: Marqus et al., 2017; Fernández Massó et al., 2013; Daghero et al., 2020
