Ponente
Descripción
Neisseria meningitidis causes meningococcal disease, which has more incidence in children under 2 years of age and people over 65 years of age. Annually it causes 300 000 deaths and leaves one in five affected with devastating long-term sequelae. Conjugate vaccines, based on the bound of the polysaccharide capsule of the bacteria to a carrier protein, are a preventive, safe and effective treatment for this disease. In the chemical conjugation reaction of these biomolecules by the 1,3-dipolar cycloaddition method, the use of Cu(I) catalysts is required. The objective of this work is to synthesize magnetic nanocatalysts with potential applications in the 1,3-dipolar cycloaddition for the conjugation of the polysaccharide MenW135 and the TT carrier protein. For this, two systems were obtained: the first is a hybrid iron and Cu(I) nanocomposite and the second is iron oxide nanoparticles synthesized by the coprecipitation method, with subsequent silica coating and Cu(I) deposition. These systems were characterized by different chemical-physical techniques such as: SEM, TEM-EDX, FT-IR and DRX. The catalysts were used in the chemical conjugation reaction, for which a study of their concentration and reaction time was carried out. The conjugates obtained were characterized by High Performance Size Exclusion Chromatography, colorimetric techniques and 1H-NMR. Magnetic nanocatalysts were obtained with suitable characteristics for their use in the Huisgen 1,3-dipolar cycloaddition reaction. Optimal conditions were established for the conjugation reaction. Glycoconjugates were obtained with suitable chemical-physical characteristics.
