Ponente
Descripción
Use of magnetic separation has been proposed as an alternative approach in the process of simplification matrices of tetanus toxoid-Streptococcus pneumoniae polysaccharide conjugate. The development of an efficient methodology for the separation of tetanus toxoid protein in conjugate samples for analytical purposes depends on three fundamental factors: obtaining magnetic support material, analyte characteristics and separation conditions. In this work, iron oxide nanoparticles were obtained to study the separation behavior of tetanus toxoid in water. Magnetite nanoparticles functionalized with polyethylene glycol, (3 Aminopropyl) triethoxysilane, adipic acid di-hydrazide, polyacrylic acid, gallic acid were synthesized by coprecipitation and post-synthesis coating. The nanoparticles were characterized by XRD, FT-IR, VSM, TEM and the aqueous suspension behavior was studied by DLS and Zeta Potential. The charge distribution of the protein in water was studied in a pH range, and the isoelectric point of was determinate by Electrophoretic Light Scattering. The nanoparticles modified with gallic and polyacrylic acid showed the highest stability in water and the most homogeneous distribution of hydrodynamic diameters. Tetanus toxoid adsorption capacity was calculated for each nanoparticle system at two different pH. The systems with amino functional groups showed higher adsorption capacity values than the nanoparticles functionalized with carboxyl groups at both pH.
